Long-term culture and high-resolution microscopy of spheroids, organoids, and suspension cells on a non-adherent surface
- Superior to the standard ultra-low attachment (ULA) surfaces: stable, biologically inert surface for long-term experiments without any cell or biomolecule adhesion
- Excellent microscopic quality without any autofluorescence: 100% surface passivation combined with the highest optical quality of the ibidi Polymer Coverslip
Applications
- Generation and long-term culture of spheroids and organoids without scaffold
- High resolution fluorescence microscopy of organoids, spheroids, embryoid bodies (EBs), and cells in suspension
- Background-free analysis of cell-cell interactions
- Prevention of stem cell differentiation due to attachment
- Culture of suspension cells in a permanently unattached state
- Self-assembly tumor spheroid formation assays
- 3D tumor spheroid models
Technical Features
- µ-Dish 35 mm, high with non-adherent, passivated Bioinert surface
- Ready-to-use without prior surface treatment or preparation
- 200 nm hydrophilic polyol hydrogel—stable and covalently bound
- No adsorption, coating, or binding of proteins, antibodies, enzymes, and other biomolecules
- Compatible with all fixatives and staining solutions
- No autofluorescence
- Non-cytotoxic, biologically inert, and non-degradable
- Compatible with immersion oil
