Solis BioDyne Oö

SolisFAST® Master Mix with UNG

  • PCR Results in 20 minutes
  • analyze one or multiple targets per reaction
  • contains dUTP and UNG to prevent carryover contamination
  • reliable results in Sanger sequencing applications
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    Description

    SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA).

    For extra convenience the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover contamination.

    Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up. 

    Our SolisFAST® Master Mix works the fastest with Eppendorf Mastercycler X50s and Eppendorf Fast PCR Tube Strips. Find out more HERE and HERE.

    Mix Properties

    Concentration: 5x

    Hot-start: yes,  2 minutes initial incubation at 98 °C

    Ready-to-load: no 

    Cloning Type: T/A cloning

    Amplicon Size: up to 5 kb

    Targets: 1-18 targets, depending on target length

    Mix Components:

    SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase

    PCR Buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR

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