A µ-Slide with one channel and three wells for culturing cells on a 3D gel matrix with defined flow
Applications
- Culture of adherent cells on a 3D gel matrix under flow conditions
- Transendothelial migration studies under flow conditions
- Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
- Simulation of blood vessels with endothelial cell culture on a soft substrate
- Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
- Apical-basal cell polarity assays
- Cell barrier model assays with apical-basal gradients
- Cell boundary assays without filters or porous membranes
- Rolling and adhesion assays using leukocytes
- Inflammation studies
- Transport studies
Technical Features:
(e.g., Collagen Type I, Matrigel, Fibrinogen)
Specifications
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 3 |
| Volume of wells | 16 μl |
| Well dimensions | 5.4 mm x 4.0 mm |
| Well height (without channel) | 0.8 mm |
| Growth area per well | 0.21 cm² |
| Coating area per well | 0.34 cm² |
| Channel width | 5.0 mm |
| Channel volume (without wells) | 150 μl |
| Channel height (without well) | 0.6 mm |
| Adapters | Female Luer |
| Volume per reservoir | 60 μl |
| Top cover: | ibidi Polymer Coverslip |
| Bottom: | ibidi Polymer Coverslip |
Click here to access the ;ibidi Collagen Calculator, a free software tool, designed to simplify the process of creating collagen gel protocols.
