µ-Slide Spheroid Perfusion

A perfusable channel µ-Slide with 3 x 7 wells for long-term spheroid cultivation and high-end microscopy

• Ideal for the long-term cultivation and perfusion of 3D spheroids or organoids
• Flexible preparation for perfusion: spheroids can be transferred or created directly in the wells
• Imaging chamber with excellent optical quality for high-resolution microscopy

Applications

• Cultivation and high-resolution microscopy of three-dimensional cell aggregates (e.g., spheroids and organoids)
• For use with the ibidi Pump System or any other pump device with Luer connectors
• Perfusion of spheroids for fresh medium supply during long-term cultivation and access to spheroid growth kinetics
• Spheroid generation directly in the wells
• Spheroid retrieval for downstream processing (e.g., immunofluorescence, histology, and biochemical assays)
• Perfusion of cell monolayers (adherent cells), suspension cells, or co-cultures
• Organ-on-a-chip setups with up- and downstream metabolization
• Live cell imaging and microscopy of 3D cell aggregates
• Immunofluorescence staining and high-resolution fluorescence microscopy of living and fixed cells and cell aggregate

Technical Features

• Optimized well geometry for spheroid/organoid culture and microscopy
• Analysis of up to 21 samples in one slide
• Perfusion can be applied for optimal nutrient supply during the cultivation of 3D cell aggregates
• Initial open well format allows for easy sample preparation—easily close the wells with a coverslip for the application of flow
• Channels create a simple fluidic connection of the wells
• Luer adapters enable easy pump connection (e.g., to the ibidi Pump System)
• Available with three surfaces:
  • Bioinert for complete surface passivation and no cell adhesion
  • Uncoated for minimal cell adhesion and suspension cells
  • ibiTreat (tissue culture-treated) for optimal cell adhesion
• Sample can be observed through the ibidi Polymer Coverslip bottom using high-resolution fluorescence microscopy
• Compatible with staining and fixation solutions
• Compatible with differential interference contrast (DIC) microscopy when used with a DIC lid
• Made of fully biocompatible materials