5x HOT FIREPol® Multiplex qPCR Mix (ROX)
For in vitro use only
Description:
HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and ROX passive reference dye according to system requirements. HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Reagents Provided:
- HOT FIREPol® DNA Polymerase
- 5x Multiplex qPCR buffer
- 15 mM MgCl2, 1x PCR solution – 3 mM MgCl2
- dNTPs, including dUTP The mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix and must be purchased separately.
- ROX dye If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Shipping and Storage Conditions:
Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
