SolisFAST® Master Mix, Ready to Load

Mix Properties

Concentration: 5x

Hot-start: yes,  2 minutes initial incubation at 98 °C

Ready-to-load: yes, includes two loading dyes

Cloning Type: T/A cloning

Amplicon Size: up to 5 kb

Targets: 1-18 targets, depending on target length

Mix Components:

SolisFAST^® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.

PCR buffer: Includes 17.5 mM MgCl₂ (1x PCR solution 3.5 mM MgCl₂)_, dNTPs (dATP, dCTP, dGTP, dTTP),  additives that maximize efficiency of PCR

Green loading dye¹: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.

¹The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).