Home … All Products Molecular & Cell Biology PCR and qPCR reagents cDNA synthesis Reverse Transcriptases FIREScript® KIT
FIREScript® KIT
Solis BioDyne Oö

FIREScript® KIT

  • high specificity and yield
  • wide reaction temperature from 37°C to 60°C
  • fast 15 min reaction time
  • a simple 2-vial kit with enzyme and reaction buffer only
  • reaction set-up and shipment without ice
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    £122.30 VAT
    Ex. Inc.
    UNSPSC: 41106313
    Manufacturer Part No: 06-13-00050
    Pack Size: 50

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    Description

    FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.

    This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.

    FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

    Applications

    • First-strand cDNA synthesis
    • RT-PCR
    • RT-qPCR

    Properties

    Sample type: RNA

    Final product: cDNA (first strand)

    Product format: Enzyme and reaction buffer (separate tubes)

    Enzyme: FIREScript® Reverse Transcriptase

    Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.

    Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.

    Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

    Reagents

    FIREScript® Reverse Transcriptase (200 U/μl)

    10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)

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