FIREScript® RT cDNA synthesis MIX with Random primers
Description
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.
This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Applications
- First-strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme mix with all reagents included
Enzyme: FIRESript® Reverse Transcriptase
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.
Unit description: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(rA)•oligo(dT) as a template in a total reaction volume of 50 μl.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
FIREScript® Enzyme Mix (FIREScript® RT and RiboGrip® RNase inhibitor)
10x RT Reaction Premix without primers
RT reaction buffer with DTT and dNTPs
10x RT Reaction Premix with Random primers
RT reaction buffer with DTT, dNTPs and Random primers
10x RT Reaction Premix with Oligo (dT) primer
RT reaction buffer with DTT, dNTPs and Oligo (dT)
10x RT Reaction Premix with Oligo (dT) and Random primers RT reaction buffer with DTT, dNTPs, Oligo (dT) primer and Random primers
Water, nuclease free
