Description
HOT FIREPol® MultiPlex Mix is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water).
Generated PCR products are compatible with TA cloning procedures
HOT FIREPol® MultiPlex Mix with 10 mM MgCl2
Properties
Concentration: 5x
Hot-start: yes, initial activation in 12-15 min
Ready to load: no
Fidelity: 1 x Taq
Cloning Type: T/A cloning
Multiplexing: up to 18 targets (depending on amplicon length)
Amplicon Size: up to 5 kb
Mix Components
- HOT FIREPol® DNA polymerase
- 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution – 2 mM MgCl2)
- dNTPs
- BSA
- Compound that increases sample density (loading dyes not included)
Variant
Pack/Size
Qty
HOT FIREPol® MultiPlex Mix with 10 mM MgCl2 - 10 - ml (10-pack: 10 x 1 ml; 2500 rxn)
10 ml (10-pack: 10 x 1 ml; 2500 rxn) / Pack
HOT FIREPol® MultiPlex Mix with 10 mM MgCl2 - 5 - ml (5-pack: 5 x 1 ml; 1250 rxn)
5 ml (5-pack: 5 x 1 ml; 1250 rxn) / Pack
