Description
RiboGrip® RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A, RNase B and RNase C.
RiboGrip® inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. It is primarily used to prevent RNA degradation by contaminating RNases in various assays that use RNA sample materials, such as first strand cDNA synthesis, RT-(q)PCR, RT-LAMP, etc.
RiboGrip® also includes a genetic modification - Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology [1]. Stability TAG makes RiboGrip® extremely tolerant to higher temperatures and enables room temperature shipping as well as effective use in assays requiring high incubation temperatures. Demonstrated through our in-house testing where RiboGrip® retains full activity after 1 hour incubation at 60 °C.
Unique high concentration and glycerol free formulation is perfect for lyophilization! Click HERE to see our lyo-compatible RiboGrip®.
Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits.
Our RiboGrip® is also compatible with Illumina's protocols (particularly single-cell sequencing). See HERE.
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
Applications
Preventing RNA degradation in variety of applications including:
- First-strand cDNA synthesis
- RT-PCR, RT-qPCR, RT-LAMP
- In vitro transcription and translation
- RNA isolation and purification
- RNA sequencing
