Description
SOLIScript® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.
Applications
- Detection and quantification of RNA and DNA targets*
- DNA/LNA hydrolysis probe-based assays
- Suitable for singleplex and multiplex reactions (up to 5 plex)
* Read more from the application note.
