Solis BioDyne Oö

SOLIScript RT cDNA synthesis KIT

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SOLIScript RT cDNA synthesis KIT

For in vitro use only

Description:

SOLIScript RT cDNA Synthesis Kit contains all the reagents you need to synthesize cDNA in 6 separate vials. Its fast reaction speed allows you to perform your synthesis in just 30 minutes while the high working temperature of the enzyme (up to 60 °C) ensures high yield and great sensitivity. The kit format allows flexibility including the preincubation of complex RNA.

Applications:

- First strand cDNA synthesis

- RT-PCR

- RT-qPCR

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 °C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

- SOLIScript Reverse Transcriptase

- RiboGrip RNase Inhibitor

- 10x RT Reaction Buffer with DTT

500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT

- Oligo (dT) Primer

- dNTP Mix

- Water, nuclease free

Shipping and Storage Conditions:

Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

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