SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme mix with all reagents included
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
SOLIScript® Enzyme Mix with SOLIScript® RT and RiboGrip® RNase Inhibitor
10x RT Reaction Premix without primers, RT reaction buffer with DTT and dNTPs
Water, nuclease free
